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Cellular respiration – measuring energy production in plants

University: California State University, Northridge

Course: Biological Principles II and Lab (BIOL 107/L)

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Date: 9/16/2020

Title: Cellular Respiration- Measuring Energy Production in Plants

Purpose: The lab’s purpose is to compare the volume of oxygen consumed by germinating and

nongerminating soybean seeds in order to estimate the amount of cellular respiration occurring.


– germinating soybeans

– nongerminating soybeans

– glass beads

– polyester fluffs

– cotton balls

– respirometer

– test tubes

– potassium hydroxide

– spoon


We took notes on the volume changes (mL) in each tube with germinating soybeans,

nongerminating soybeans with glass beads, and glass beads at 10-minute intervals for 20 minutes



Tube 1- Germinating Soybeans had the largest change in O2 volume, followed by Tube 2-

Nongerminating Soybeans and then Tube 3- Glass Beads. In addition, Tube 2 had the most O2

throughout the entire experiment while Tube 1 nearly consumed all of the O2. The glass bead tube

was a negative control as it did not produce any desired or expected results. The average oxygen

consumption rate for the germinating soybeans was 0.24 mL/min and 0.02 mL/min for the

nongerminating soybeans.

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