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Cellular respiration – measuring energy production in plants
University: California State University, Northridge
Course: Biological Principles II and Lab (BIOL 107/L)
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LAB 5
Date: 9/16/2020
Title: Cellular Respiration- Measuring Energy Production in Plants
Purpose: The lab’s purpose is to compare the volume of oxygen consumed by germinating and
nongerminating soybean seeds in order to estimate the amount of cellular respiration occurring.
Materials:
– germinating soybeans
– nongerminating soybeans
– glass beads
– polyester fluffs
– cotton balls
– respirometer
– test tubes
– potassium hydroxide
– spoon
Observations:
We took notes on the volume changes (mL) in each tube with germinating soybeans,
nongerminating soybeans with glass beads, and glass beads at 10-minute intervals for 20 minutes
total.
Analysis:
Tube 1- Germinating Soybeans had the largest change in O2 volume, followed by Tube 2-
Nongerminating Soybeans and then Tube 3- Glass Beads. In addition, Tube 2 had the most O2
throughout the entire experiment while Tube 1 nearly consumed all of the O2. The glass bead tube
was a negative control as it did not produce any desired or expected results. The average oxygen
consumption rate for the germinating soybeans was 0.24 mL/min and 0.02 mL/min for the
nongerminating soybeans.
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